Genetic classification of severe early childhood caries by use of subtracted DNA fragments from Streptococcus mutans.

Genetic Classification of Severe Early Childhood Caries Using 1 Subtracted DNA

1 Streptococcus mutans is one of several members of the oral indigenous biota linked with severe 2 early childhood caries (S-ECC). Because most humans harbor S. mutans, but not all manifest 3 disease, it has been proposed that the strains of S. mutans associated with S-ECC are 4 genetically distinct from those found in caries free (CF) children. The objective of this study was 5 to identify com...

The Frequency of Streptococcus Mutans and Lactobacillus spp.in 3-5-year- old Children with and without Dental Caries

Abstract Background and Objective: The high occurrence of early childhood caries (ECC) is one of the most common problems in children dentistry. Streptococcus mutans and lactobacilli.spp are closely associated with the development of early childhood caries (ECC). Therefore, the aim of this study was to investigate the frequency of streptococcus mutans and lactobacilli.spp in 3-5 –year- old ch...

Identification of unique bacterial gene segments from Streptococcus mutans with potential relevance to dental caries by subtraction DNA hybridization.

Using DNA subtractive hybridization, 49 unique gene segments were identified from a strain of Streptococcus mutans that was isolated from a patient with severe early childhood caries (S-ECC). Further hybridization with DNA from other S. mutans strains isolated from both caries-active and caries-free subjects yielded five unique sequences of DNA common to strains associated with S-ECC.

Clonal analysis of the microbiota of severe early childhood caries.

BACKGROUND/AIMS Severe early childhood caries is a microbial infection that severely compromises the dentition of young children. The aim of this study was to characterize the microbiota of severe early childhood caries. METHODS Dental plaque samples from 2- to 6-year-old children were analyzed using 16S rRNA gene cloning and sequencing, and by specific PCR amplification for Streptococcus mut...

The Comparison of Proteins Elaborated by Streptococcus mutans Strains Isolated from Caries Free and Susceptible Subjects