Diagnostic molecular pathology, part 2: proteomics and clinical applications of molecular diagnostics in hematopathology.

Blastic Plasmacytoid Dendritic Cell Neoplasm; A Report of Three Cases

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematodermic myeloid malignancy that is known to be derived from plasmacytoid dendritic cells which are characterized by expression of CD4, CD56, and more specific markers such as CD123. Here, the authors present three cases of BPDCN diagnosed in the past two years and address different available diagnostic modalities such as morpho...

Cytoplasmic Her2/neu immunohistochemical staining in breast cancer; From a molecular point of view

Applications of flow cytometry and immunohistochemistry to diagnostic hematopathology.

OBJECTIVE Diagnostic hematopathology depends on the applications of flow cytometric immunophenotyping and immunohistochemical immunophenotyping combined with the cytomorphology and histologic features of each case. Select cases may require additional ancillary cytogenetic and molecular studies for diagnosis. The purpose of this review is to focus on the applications of flow cytometric and immun...

Variations in pre-analytical FFPE sample processing and bioinformatics: challenges for next generation molecular diagnostic testing in clinical pathology

Molecular diagnostics within cellular pathology have been performed since the late 1990s and have developed to include a range of techniques including short tandem repeat (STR) identity analysis, classifi cation of tumours and clonality determinations in hematopathology. More recently, with the introduction of qPCR and more recently of next generation sequencing (NGS) as shown in Figure 1, prec...

Evaluation of JAK2 (V617F) Mutation in Iranian Veterans with Delayed Complications of Sulphur Mustard Poisoning

Background: Sulfur mustard was the most widely applied chemical warfare agent by the Iraqi army in Iran–Iraq war (1983-1988). Considering the role of sulfur mustard toxicity in hematopoietic neoplasms and also new role of JAK2 mutation in these neoplasms, we assessed this mutation and delayed hematologic complications in veterans exposed to sulfur mustard. Methods: This case control st...