Molecular cloning of a cDNA for the developmentally regulated brain protein, drebrin.
نویسندگان
چکیده
A lambda gt11 cDNA library from 10-day-old chicken embryo was screened immunologically using an antiserum against drebrins E1, E2 and A, proteins previously designated S5, S6 and S54, respectively. A cDNA clone for a common domain of drebrin was isolated. Northern blot analysis of chicken brain indicated that drebrin mRNAs are about 2.7 kilobases in molecular size and that expression of these proteins is developmentally regulated.
منابع مشابه
Molecular cloning of a developmentally regulated brain protein, chicken drebrin A and its expression by alternative splicing of the drebrin gene.
Drebrins are developmentally regulated proteins found in the chicken brain and are classified into three forms, E1, E2 and A. Previously we isolated two cDNAs corresponding to the embryonic drebrin mRNAs from a chick embryo cDNA library. They differed in that an internal 129-nucleotide sequence, designated ins1, was inserted in the cDNA encoding drebrin E2 and was deleted in the other cDNA enco...
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ورودعنوان ژورنال:
- Brain research
دوره 464 1 شماره
صفحات -
تاریخ انتشار 1988