Investigation of membrane protein-protein interactions using correlative FRET-PLA.
نویسندگان
چکیده
Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein-protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein-protein interactions.
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ورودعنوان ژورنال:
- BioTechniques
دوره 57 4 شماره
صفحات -
تاریخ انتشار 2014