Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus.
نویسندگان
چکیده
Recent data show that more than 50% of catheter-associated bloodstream infections are caused by staphylococci. Staphylococcal infections produced by intercellular-adhesion cluster (ica) carriers can be even more problematic due to the presence of methicillin and mupirocin resistance genes. In the present study, a multiplex PCR protocol that allows the simultaneous identification of staphylococci and detection of both the ica and methicillin and/or mupirocin resistance genes was developed. Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis.
منابع مشابه
بررسی فنوتیپ و ژنوتیپ مقاومت به موپیروسین در بین استافیلوکوک های مقاوم و حساس به متی سیلین جدا شده از عفونت های بیمارستانی
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متن کاملشناسایی مقاومت به موپیروسین در سویه‌های استافیلوکوکوس اورئوس جدا شده از بیماران 4 بیمارستان دانشگاهی تهران با روش PCR
Introduction: Mupirocin, an inhibitor of bacterial isoleucyl-tRNA synthetase, has used to treat staphylococcal skin infections as well as to eliminate nasal carriage of Staphylococcus aureus. Mupirocin resistant Staphylococcus aureus strains are seen worldwide but there is no report from Iran. In the present study, by using phenotyping methods (disk diffusion and E-test) in combination with P...
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ورودعنوان ژورنال:
- International microbiology : the official journal of the Spanish Society for Microbiology
دوره 7 1 شماره
صفحات -
تاریخ انتشار 2004