Npgrj_Nprot_339 2315..2319
نویسندگان
چکیده
Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo ‘‘unlimited’’ division. Clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing radiation, but can also be used to determine the effectiveness of other cytotoxic agents. Only a fraction of seeded cells retains the capacity to produce colonies. Before or after treatment, cells are seeded out in appropriate dilutions to form colonies in 1–3 weeks. Colonies are fixed with glutaraldehyde (6.0% v/v), stained with crystal violet (0.5% w/v) and counted using a stereomicroscope. A method for the analysis of radiation dose–survival curves is included.
منابع مشابه
Phosphine and tertiarybutylphosphine adsorption on the indium - rich InP ( 001 ) - ( 2 · 4 ) surface
Phosphine and tertiarybutylphosphine adsorption on the indium-rich InP (001)-(2 · 4) surface at 25 C have been studied by internal reflection infrared spectroscopy, X-ray photoelectron spectroscopy, and low energy electron diffraction. Both molecules form a dative bond to the empty dangling bonds on the In–P heterodimers and the second-layer In–In dimers and vibrate symmetrically at 2319 (2315)...
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