AGI June 39/6
نویسنده
چکیده
Xie, Qing-Mei, Jian-Su Shao, and David H. Alpers. Rat intestinal a1-antitrypsin secretion is regulated by triacylglycerol feeding. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G1452–G1460, 1999.—a1-Antitrypsin (AAT) is secreted by the enterocyte, but its regulation of expression, intramucosal distribution, and functional status are unclear. After corn oil gavage (plus Pluronic L-81 to block chylomicron release), rat intestine was examined for mRNA encoding AAT, immunoreactivity by light and electron microscopy, and protein content by Western blot. Species-specific antisera used were raised against both AAT and surfactant-like particle (SLP), a membrane secreted by the enterocyte in response to fat feeding. Purified luminal SLP was fractionated by Bio-Gel P-200 chromatography to assess its interaction with AAT. Triacylglycerol feeding maximally increased mucosal mRNAencoding AAT and AAT intracellular protein content by 3 and 5 h, respectively. Immunocytochemistry revealed predominance of AAT in basolateral spaces around enterocytes and Pluronic-blocked extracellular accumulation of AAT, patterns nearly identical to those of secreted SLP. About 10% of AAT was reversibly associated with SLP. LuminalAAT was smaller (51 kDa) than mature AAT (55 kDa) and did not form a complex with pancreatic elastase. When the common bile duct was tied, excluding pancreatic proteases from the lumen, mature AAT that was cleaved by pancreatic elastase was secreted. The luminal secretion of AAT and its reversible association with SLP suggest an intracellular association and a possible role for AAT during lipid digestion and absorption.
منابع مشابه
AGI June 39/6
Muscará, Marcelo N., and John L. Wallace. Nitric Oxide. V. Therapeutic potential of nitric oxide donors and inhibitors. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G1313–G1316, 1999.—Nitric oxide is a crucial mediator of gastrointestinal mucosal defense, but, paradoxically, it also contributes to mucosal injury in several situations. Inhibitors of nitric oxide synthesis and compounds...
متن کاملAGI Apr. 39/4
J. M. ANDREWS,1 H. NATHAN,2 C. H. MALBERT,3 M. A. M. T. VERHAGEN,4 M. GABB,5 G. S. HEBBARD,1 D. KILPATRICK,6 S. MACDONALD,2 C. K. RAYNER,1 S. DORAN,1 T. OMARI,7 E. O’YOUNG,2 C. FRISBY,2 R. J. FRASER,1 M. SCHOEMAN,2 M. HOROWITZ,1 AND J. DENT2 Departments of 1Medicine, 2Gastrointestinal Medicine, and 5Radiology, Royal Adelaide Hospital, and 7Department of Gastroenterology, Women’s and Children’s ...
متن کاملElectroacupuncture decreases the urinary bladder pressure in patients with acute gastrointestinal injury.
The present study investigates the effects of electroacupuncture (EA) on urinary bladder pressure (UBP) in patients with acute gastrointestinal injury (AGI). Twenty patients with AGI admitted to the First Hospital of Jiaxing intensive care unit from December 2011 to June 2013 were evaluated. Conventional group patients (n = 10) were administered moderate enteral nutritional support, and electro...
متن کاملAGI Apr. 39/4
Nevière, R. R., M. L. Pitt-Hyde, R. D. Piper, W. J. Sibbald, and R. F. Potter. Microvascular perfusion deficits are not a prerequisite for mucosal injury in septic rats. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G933–G940, 1999.—Our major objective was to investigate whether injury to the mucosa of the small intestine occurred in a normotensive model of sepsis and whether such inju...
متن کاملAGI Apr. 39/4
Simmons, James G., Jolanta B. Pucilowska, and P. Kay Lund. Autocrine and paracrine actions of intestinal fibroblast-derived insulin-like growth factors. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G817–G827, 1999.— Paracrine and autocrine actions of the insulin-like growth factors (IGFs) are inferred by local expression within the bowel. CCD-18Co cells, IEC-6 cells, and immunoneutral...
متن کاملThe association between farming activities, precipitation, and the risk of acute gastrointestinal illness in rural municipalities of Quebec, Canada: a cross-sectional study
BACKGROUND Increasing livestock density and animal manure spreading, along with climate factors such as heavy rainfall, may increase the risk of acute gastrointestinal illness (AGI). In this study we evaluated the association between farming activities, precipitation and AGI. METHODS A cross-sectional telephone survey of randomly selected residents (n = 7006) of 54 rural municipalities in Que...
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