Enzyme-immunoassay for determination of progesterone in milk from cows.
نویسندگان
چکیده
An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0.1-20 pmol, i.e. 0.03-0.63 ng and intra- and inter-assay coefficients of variation of less than 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0.98) for milk samples from cows during the oestrous cycle and early pregnancy.
منابع مشابه
Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay
Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commerc...
متن کاملPretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows' milk.
New rapid method of lateral flow enzyme immunoassay (LFEIA) for progesterone detection in whole cows' milk was developed. The test system utilized horseradish peroxidase as a label along with the substrate solution containing 3,3',5,5'-tetramethylbenzidine and dextran sulfate to obtain an insoluble blue colored product of the enzyme reaction on a surface of analytical membrane (test and control...
متن کاملPregnancy diagnosis in dairy goats and cows using progesterone assay kits.
Early pregnancy diagnosis in dairy goats and cows was studied using enzyme immunoassays (EIA) to measure progesterone concentrations in whole milk samples collected approximately 3 weeks after mating. Two qualitative on-farm assay kits and 2 quantitative assay kits, all designed for use in the dairy cow, were tested for their accuracy with goats milk samples. Accuracy of diagnosis of goat pregn...
متن کاملTeaching in the Laboratory Progesterone in milk: a simple experiment illustrating the estrous cycle and enzyme immunoassay
Rioux, Pierre, and Denis Rajotte. Progesterone in milk: a simple experiment illustrating the estrous cycle and enzyme immunoassay. Adv Physiol Educ 28: 64–67, 2004; 10.1152/advan.00048.2002— Experiments designed for students in reproductive physiology are rare. Here, we describe a simple experiment concerning a physiological aspect of the reproductive system. Milk samples are obtained from cows...
متن کاملAccuracy of oestrus detection in cows and its economic impact on Shiraz dairy farms
No report has been published on the economical losses associated with errors in oestrus detection in cows under Iranian intensive dairy management. The following study was carried out to determine the accuracy of oestrus detection by farmers using artificial insemination in Shiraz dairy farms. Milk samples were collected from 179 Holstein milking cows at the time of artificial insemination. Mil...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of reproduction and fertility
دوره 62 1 شماره
صفحات -
تاریخ انتشار 1981