Identification of importin alpha 7 specific transport cargoes using a proteomic screening approach

نویسندگان

  • Stefanie Hügel
  • Reinhard Depping
  • Gunnar Dittmar
  • Franziska Rother
  • Ryan Cabot
  • Matthias D. Sury
  • Enno Hartmann
  • Michael Bader
چکیده

This research was originally published in Molecular & Cellular Proteomics. Huegel, S. and Depping, R. and Dittmar, G. and Rother, F. and Cabot, R. and Sury, M.D. and Hartmann, E. and Bader, M. Identification of importin alpha 7 specific transport cargoes using a proteomic screening approach. Mol Cell Proteomics; 2014, 13:1286-1298. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structural basis for the nuclear protein import cycle.

Transport of macromolecules between the nuclear and cytoplasmic compartments through NPCs (nuclear pore complexes) is mediated by soluble transport factors that are commonly members of the importin-beta superfamily. In the nuclear protein import cycle, importin-beta binds cargo in the cytoplasm (usually via the importin-alpha adaptor) and transports it through NPCs with which it interacts trans...

متن کامل

The classical nuclear localization signal receptor, importin-alpha, is required for efficient transition through the G1/S stage of the cell cycle in Saccharomyces cerevisiae.

There is significant evidence linking nucleocytoplasmic transport to cell cycle control. The budding yeast, Saccharomyces cerevisiae, serves as an ideal model system for studying transport events critical to cell cycle progression because the nuclear envelope remains intact throughout the cell cycle. Previous studies linked the classical nuclear localization signal (cNLS) receptor, importin-alp...

متن کامل

Importin β contains a COOH-terminal nucleoporin binding region important for nuclear transport

Proteins containing a classical NLS are transported into the nucleus by the import receptor importin beta, which binds to cargoes via the adaptor importin alpha. The import complex is translocated through the nuclear pore complex by interactions of importin beta with a series of nucleoporins. Previous studies have defined a nucleoporin binding region in the NH2-terminal half of importin beta. H...

متن کامل

Single-molecule measurements of importin alpha/cargo complex dissociation at the nuclear pore.

Macromolecules are transported between the cytoplasm and the nucleoplasm of eukaryotic cells through nuclear pore complexes (NPCs). Large (more than approximately 40 kDa) transport cargoes imported into the nucleus typically form a complex with at least one soluble transport cofactor of the importin (Imp) beta superfamily. Many cargoes require an accessory cofactor, Imp alpha, which binds to Im...

متن کامل

Extensive cargo identification reveals distinct biological roles of the 12 importin pathways

Vast numbers of proteins are transported into and out of the nuclei by approximately 20 species of importin-β family nucleocytoplasmic transport receptors. However, the significance of the multiple parallel transport pathways that the receptors constitute is poorly understood because only limited numbers of cargo proteins have been reported. Here, we identified cargo proteins specific to the 12...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017