Isolation of disaturated phosphatidylcholine with osmium tetroxide.
نویسندگان
چکیده
A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 17 3 شماره
صفحات -
تاریخ انتشار 1976