Rotavirus 993/83, isolated from calf faeces, closely resembles an avian rotavirus.
نویسندگان
چکیده
Polypeptides from purified virions of the calf rotavirus (RV) isolate 993/83 and those from the pigeon RV isolate PO-13 comigrated on SDS-polyacrylamide gels. Two polypeptides of 45K and 47K were detected at the position of VP6. Both proteins behaved like authentic VP6 protein with EDTA and heat treatment. RV 993/83 and PO-13 showed identical one-dimensional peptide maps for VP2, and the 45K and 47K proteins. More than 70% of sera from German cattle older than 1 year showed neutralizing serum antibodies to RV 993/83 and RV PO-13.
منابع مشابه
Isolation of an avianlike group A rotavirus from a calf with diarrhea.
An atypical group A rotavirus (993/83) was isolated from a 3-day-old German calf with diarrhea. It differed from 35 conventional German bovine rotavirus isolates analyzed previously with respect to subgroup (strain 993/83 was non-subgroup I and non-subgroup II), serotype (strain 993/83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and electropher...
متن کاملDetection of avian-like rotavirus A VP4 from a calf in Japan
A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen....
متن کاملThe dsRNA Electrophoretype of Some Isolated Iranian Calf Rotaviruses
A rapid and simple technique for diagnosis of rotaviral diarrhea is polyacrylamide gel electrophoresis (PAGE) of dsRNA extracted from fecal samples. To determine the electrophoretype of Iranian calf rotavirus, 23 rotaviral positive fecal samples from diarrheic Holstein calves at the age of less than one month in Tehran region were examined. The viral dsRNA was extracted with phenol-chloroform, ...
متن کاملThe isolation and cultivation of calf rotavirus in the Republic of South Africa.
Calf rotavirus was cultivated and propagated in tissue culture from faeces of 3-week-old calves suffering from severe diarrhoea. Criteria for viral involvement were: production of cytopathic effect in primary foetal calf kidney cells, specific fluorescence, and identification of the agent by means of electron microscopy. In a limited serological survey the majority of the cows on an infested fa...
متن کاملAn antigenic subunit present in rotavirus infected faeces.
It has been found by immune electron microscopy that rotavirus-infected faeces, calf or human, contain an antigenic subunit associated with the inner of the two virus capsids. This internal component represents the group specific antigen for the rotavirus group and the subunit reacts with both homologous and heterologous antiserum. It can therefore be used in diagnostic tests and in this paper ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of general virology
دوره 73 ( Pt 7) شماره
صفحات -
تاریخ انتشار 1992