Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.
نویسندگان
چکیده
To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 80 2 شماره
صفحات -
تاریخ انتشار 2010