Value of the concomitant use of complement fixation and immunodiffusion tests in the diagnosis of coccidioidomycosis.
نویسندگان
چکیده
Complement fixation titers of 1:2 and 1:4 with coccidioidin are not always of diagnostic significance. The concurrent use of the complement fixation and immunodiffusion tests is an effective means for specific serologic diagnosis of coccidioidomycosis in patients with low levels of complement-fixing antibodies. In routine testing, it is recommended that only sera that are negative for complement-fixing antibodies at 1:8 but positive in the immunodiffusion test with coccidioidin be selected for titration at the 1:2 and 1:4 levels.
منابع مشابه
Coccidioidomycosis with cutaneous manifestation of erythema nodosum in Taiwan
Coccidioidomycosis is a fungal disease endemic to the southwestern USA, northern Mexico, and focal areas in Central and South America, but it is rarely reported in Taiwan. We report a case of coccidioidomycosis of probable pulmonary origin in a young woman, who came back to Taiwan after a 10-month stay in the Central Valley of California. Coccidioidomycosis was suspected by typical constellatio...
متن کاملCharacterization of a serodiagnostic complement fixation antigen of Coccidioides posadasii expressed in the nonpathogenic Fungus Uncinocarpus reesii.
Coccidioides spp. (immitis and posadasii) are the causative agents of human coccidioidomycosis. In this study, we developed a novel system to overexpress coccidioidal proteins in a nonpathogenic fungus, Uncinocarpus reesii, which is closely related to Coccidioides. A promoter derived from the heat shock protein gene (HSP60) of Coccidioides posadasii was used to control the transcription of the ...
متن کاملThe coccidioidal complement fixation and immunodiffusion-complement fixation antigen is a chitinase.
Culture filtrates and autolysates of Coccidioides immitis have provided suitable crude antigens for the serodiagnosis and prognosis of coccidioidomycosis. One of these, a heat-labile antigen which participates in the immunodiffusion reaction corresponding to the complement fixation reaction (IDCF), has been characterized as a 110-kDa native protein that, when subjected to reducing conditions an...
متن کاملComparison of immunodiffusion and complement fixation tests in the diagnosis of histoplasmosis.
The immunodiffusion and complement fixation tests for histoplasmosis were compared on 2,090 initial sera from 70 proven cases of histoplasmosis, 177 routine chest hospital patients, and 1,843 coal miners from outside the major endemic area for histoplasmosis. The complement fixation test using histoplasmin as antigen detected antibodies in the sera of 72.8% of the 70 proven cases, while the com...
متن کاملIndirect fluorescent-antibody and quantitative agar-gel immunodiffusion tests for the serological diagnosis of paracoccidioidomycosis.
The value of various serological tests in the diagnosis of paracoccidioidomycosis was studied. Quantitative agar-gel immunodiffusion and indirect immunofluorescent tests were performed, and the results were compared with those of complement fixation and qualitative agar-gel procedures. The quantitative immunodiffusion procedure was found to serve as the simplest and safest quantitative test tha...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 28 4 شماره
صفحات -
تاریخ انتشار 1974