Use of Monoclonal Antibodies as Diagnostic and Therapeutic Reagents in Acute Lymphoblastic Leukemia1
نویسندگان
چکیده
A monoclonal antibody specific for a common acute lymphoblastic leukemia (ALL) antigen has been generated and char acterized. This antibody (J-5) is reactive with leukemic cells from most patients with non-T-cell ALL and some patients with chronic myelocytic leukemia in blast crisis. J-5 antibody is not reactive with leukemic cells from patients with either T-cell ALL, chronic lymphocytic leukemia, acute myeloblastic leuke mia, or stable-phase chronic myelocytic leukemia. In addition to diagnostic applications, anti-common ALL antigen mono clonal antibody has been used to study the effect of specific serotherapy in the treatment of ALL. In the first patient with ALL treated with J-5 antibody, a 90% reduction in circulating lymphoblasts occurred within 1 hr of starting antibody infusion. Despite continued serotherapy, however, lymphoblasts per sisted in peripheral blood and bone marrow. Analysis of cell surface markers during serotherapy suggested that resistance to antibody-mediated lysis in vivo may have been due to antigenie modulation of leukemic cells in response to J-5 mono
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