Background Analysis
نویسنده
چکیده
Microarray technology provides a powerful tool with which to study the coordinate expression of thousands of genes in a single hybridization reaction. However, probe specificity can have a direct influence on the accuracy of transcript quantitation. Therefore, it is essential to account for non-specific binding and cross-hybridization when analyzing data from microarray analysis. Agilent’s in situ microarrays with 25mer probes and unique control probes allow assessment of specific expression profiles of cellular transcripts. Experiments presented here show that Agilent’s 25mer probe design and expression system offer a robust platform for the analysis of specific gene expression. The experiments also demonstrate the utility of Agilent’s positive and negative controls in evaluating microarray data. In particular, two types of negative control probes allow the assessment of general background hybridization and transcript-specific cross hybridization.
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