Distinct Calcium Signaling Mechanisms in Across Lung Epithelial Monolayers by Regulation of Human Eosinophil Migration

Lung Epithelial Cells: Eosinophil Eosinophil Migration Across Monolayers of Triple Role of Platelet-Activating Factor in

Epithelial Cell Activator Chemoattractant and Priming Agent and Lung Epithelial Cells: Eosinophil Eosinophil Migration Across Monolayers of Triple Role of Platelet-Activating Factor in

Interleukin-8 plays a significant role in IgE-mediated lung inflammation.

Interleukin (IL)-8 is a potentially important cytokine in allergic respiratory responses since it is released by many resident lung cells, and it is a potent granulocyte chemoattractant. Therefore, we induced an immunoglobulin (Ig)E-mediated response in human lung samples and studied whether IL-8 was produced in sufficient quantities to promote human neutrophil and eosinophil migration across n...

Sequential migration of neutrophils across monolayers of endothelial and epithelial cells.

In the course of granulocyte-dominated lung inflammation, granulocytes migrate across the endothelium and epithelium of the lung and cause severe tissue damage. To study this process in more detail, we developed a bilayer transmigration model composed of primary human endothelial and lung epithelial cells, simultaneously cultured on opposite sides of Transwell filters. Electron microscopical an...

Integrins are Mechanosensors That Modulate Human Eosinophil Activation

Eosinophil migration to the lung is primarily regulated by the eosinophil-selective family of eotaxin chemokines, which mobilize intracellular calcium (Ca(2+)) and orchestrate myriad changes in cell structure and function. Eosinophil function is also known to be flow-dependent, although the molecular cognate of this mechanical response has yet to be adequately characterized. Using confocal fluo...