Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.
نویسندگان
چکیده
A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.
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References 1. Lahiri D, Nurnberger J. A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res 1991;19:5444. 2. Miller S, Dykes D, Polesky H. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215. 3. Sambrook J, Fritsch ER, Maniatis T, eds. Molecular cloning: a laboratory manual, 2nd ed. Col...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 47 11 شماره
صفحات -
تاریخ انتشار 1994