Effects of Caffeic Acid Incorporated into Solid L ipid Nanoparticles in Cancer Cells

Nanotechnology has great potential to make an important role in prevention of cancer, detection, diagnosis, imaging and treatment. Solid lipid nanoparticles (SLNs) have been introduced as an alternative drug carrier system. Some of the features of SLN include good tolerability, site-specific targeting, stability, high drug payload, controlled drug release. Caffeic acid (CA) is a widespread phenolic acid that occurs naturally in many agricultural products. CA has been reported to has antitumor activity and anti-inflammatory properties, inhibit certain enzyme activities. We evaluated that CA-SLN interaction with H-ras transformed cell line, 5RP7. In this study, we aim to examine morphology, structural and ultrastructural changes of 5RP7 cells which exposed to CA-SLNs find by using TEM and Confocal Microscopy. We found that CA -loaded nanoparticle formulations is significantly more effective and enhanced its efficacy against experimental tumors in the treatment of cancer as compared with free CA. K eywords: Solid lipid nanoparticles, caffeic acid, TEM, Confocal Microscopy.