Conversion of uracil and orotate to uridine 5'-phosphate by enzymes in lactobacilli.
نویسندگان
چکیده
* Predoctoral Fellow of the National Science Foundation, 1954-55; present address, Walter Reed Army Institute of Research, Washington 12, D. C. This investigation was supported by grants from the National Institutes of Health, Public Health Service, and the National Science Foundation. 1 Abbreviations used: ATP, adenosine triphosphate; 05P, orotidine 5’-phosphate; PP, inorganic pyrophosphate; PRPP, 5-phosphoribosylpyrophosphate; R5P, ribose 5-phosphate; Tris, tris(hydroxymethyl)aminomethane; U5P, uridine 5’-phosphate. 2 Kornberg, A., and Simms, E. S., unpublished observations.
منابع مشابه
Pyrimidine metabolism in Tritrichomonas foetus.
The anaerobic parasitic protozoa Tritrichomonas foetus is found incapable of de novo pyrimidine biosynthesis by its failure to incorporate bicarbonate, aspartate, or orotate into pyrimidine nucleotides or nucleic acids. Uracil phosphoribosyltransferase in the cytoplasm provides the major pyrimidine salvage for the parasite. Exogenous uridine and cytidine are mostly converted to uracil by uridin...
متن کاملProperties of pea seedling uracil phosphoribosyltransferase and its distribution in other plants.
A uracil phosphoribosyltransferase (UMP-pyrophosphorylase) was found in several angiosperms and was partially purified from epicotyls of pea (Pisum sativum L. cv. Alaska) seedlings. Its pH optimum was about 8.5; its required approximately 0.3 mm MgCl(2) for maximum activity but was inhibited by MnCl(2); its molecular weight determined by chromatography on Sephadex G-150 columns was approximatel...
متن کاملMolecular biology and biochemistry of malarial parasite pyrimidine biosynthetic pathway.
Metabolic pathways in the malarial parasite are markedly different from the host, eg, hemoglobin, fatty acids, folate and nucleic acids. Understanding of metabolic function will illuminate new chemotherapeutic targets for drug development, including the identification of target(s) for drugs in current use. The parasite-contained pyrimidine biosynthetic pathway is essential for growth and develo...
متن کاملPyrimidine Biosynthesis Is Not an Essential Function for Trypanosoma brucei Bloodstream Forms
BACKGROUND African trypanosomes are capable of both pyrimidine biosynthesis and salvage of preformed pyrimidines from the host, but it is unknown whether either process is essential to the parasite. METHODOLOGY/PRINCIPAL FINDINGS Pyrimidine requirements for growth were investigated using strictly pyrimidine-free media, with or without single added pyrimidine sources. Growth rates of wild-type...
متن کاملDevelopment of Pyrimidine-metabolizing Enzymes in Cotyledons of Germinating Peas.
Mechanisms controlling conversion of orotic acid-6-(14)C to uridine-5'-phosphate in cotyledons of germinating Alaska peas (Pisum sativum L.) were investigated. The content of 5-phosphoribosyl-1-pyrophosphate was very low in dry seeds, increased to a maximum after about 12 hours of imbibition, and then rapidly declined. Orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarbox...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 226 2 شماره
صفحات -
تاریخ انتشار 1957