In-vitro Seeding of Bladder Smooth Muscle Cells onto Bladder Acellular Matrix as Cell Delivery Vehicle in Tissue Engineering

The present study was carried out evaluated efficacy of bladder acellular matrix (BAM) as cell delivery vehicle. Smooth muscle cells were harvested by collagenase digestion of rabbit urinary bladder and the cells were cultured in growth medium comprising of 4:1 mixture of DMEM and Ham’s F-12 nutrient mixture supplemented with L-glutamine containing 100 mg/ml penicillin as well as 100 mg/ml streptomycin. Fetal bovine serum was added @ 10%. The isolated cells were cultured in-vitro to expand into sufficient number for seeding onto xenogenic bladder acellular matrix. Successful in-vitro seeding of bladder smooth muscle cells onto bladder acellular matrix was done.