The two component system BvrR / BvrS regulates the 1 expression of the Type IV secretion system VirB in 2
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چکیده
6 Carola Martínez-Núñez, Pamela Altamirano-Silva, Francisco Alvarado7 Guillén, Edgardo Moreno, Caterina Guzmán-Verri and Esteban Chaves8 Olarte 9 Programa de Investigación en Enfermedades Tropicales (PIET), Escuela de 10 Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica; 11 Escuela de Biología, Universidad Nacional, 304-3000 Heredia, Costa Rica; 12 Facultad de Farmacia, Universidad de Costa Rica, 1000 San José, Costa Rica; 13 Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, 14 1000 San José, Costa Rica; Centro de Investigación en Enfermedades 15 Tropicales, Facultad de Microbiología, Universidad de Costa Rica, 1000 San José, 16 Costa Rica; 17 18
منابع مشابه
The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins with counterparts in members of the Rhizobiaceae.
The Brucella BvrR/BvrS two-component regulatory system is homologous to the ChvI/ChvG systems of Sinorhizobium meliloti and Agrobacterium tumefaciens necessary for endosymbiosis and pathogenicity in plants. BvrR/BvrS controls cell invasion and intracellular survival. Probing the surface of bvrR and bvrS transposon mutants with monoclonal antibodies showed all described major outer membrane prot...
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BACKGROUND The two-component BvrR/BvrS system is essential for Brucella abortus virulence. It was shown previously that its dysfunction alters the expression of some major outer membrane proteins and the pattern of lipid A acylation. To determine the genes regulated by BvrR/BvrS, we performed a whole-genome microarray analysis using B. abortus RNA obtained from wild type and bvrR mutant cells g...
متن کاملBvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulence.
The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption of bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. Consequently, the role of Omp3a and Omp3b in virulence was examined. Similar to bvrS or bvrR mut...
متن کاملFunctional Characterization of the Incomplete Phosphotransferase System (PTS) of the Intracellular Pathogen Brucella melitensis
BACKGROUND In many bacteria, the phosphotransferase system (PTS) is a key player in the regulation of the assimilation of alternative carbon sources notably through catabolic repression. The intracellular pathogens Brucella spp. possess four PTS proteins (EINtr, NPr, EIIANtr and an EIIA of the mannose family) but no PTS permease suggesting that this PTS might serve only regulatory functions. ...
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Brucella organisms are pathogens that ultimate goal is to propagate in their preferred niche, the cell. Upon cell contact the bacteria is internalized via receptor molecules by activating small GTPases of the Rho subfamily and by a moderate recruitment of actin filaments. Once inside cells, Brucella localizes in early phagosomes, where it avoids fusion with late endosomes and lysosomes. These e...
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