Conventional transmission electron microscopy
نویسندگان
چکیده
Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.
منابع مشابه
Applicability of thin film phase plates in biological electron microscopy
Presented is an evaluation of phase contrast techniques in transmission electron microscopy. The traditional defocus phase contrast is compared to two recently developed phase plate techniques. One is the Zernike phase contrast transmission electron microscope, the other is the Hilbert differential contrast thransmission electron microscope. The imaging characteristics of each technique are dis...
متن کاملSynthesis and Electrochemical Study of Nano Graphitic Mesoporous Carbon
In the present study, a type of graphitic mesoporous carbon (denoted as GMC) is synthesized and its structural properties were characterized by nitrogen physisorption isotherms, transmission electron microscopy and Raman spectroscopy. According to BET and BJH methods, the specific surface area (SBET) and the average pore diameter of GMC are estimated to be 720 m2/g and 3.4 nm, respectively....
متن کاملBright-field transmission imaging of carbon nanofibers on bulk substrate using conventional scanning electron microscopy
The authors present scanning transmission electron microscopy STEM of carbon nanofibers CNFs on a bulk substrate using conventional scanning electron microscopy SEM without specimen thinning. By utilizing the electron beam tilted 85° from the substrate normal, bright-field STEM contrast is obtained for the CNFs on substrate with conventional SEM. Analysis of the observed contrast using Monte Ca...
متن کامل3D ChemiSTEMTM Tomography of Nano-scale Precipitates in High Entropy Alloys
Electron tomography is a three-dimensional technique well suited for characterizing fine-scale microstructural features on the order of 10-100 nm. Typically performed in a transmission electron microscope (TEM), it relies on successive image acquisition at multiple sample tilts. Images may be formed through various techniques including conventional and scanning transmission electron microscopy ...
متن کاملIn-Situ Lorentz Microscopy with Femtosecond Optical Illumination
Ultrafast electron microscopy as a laser-pump/ electron-probe technique allows for the investigation of structural and electronic dynamics occurring at sub-picosecond timescales and nanometer length-scales [1,2]. However, current implementations necessitate compromises in electron source brightness compared to conventional electron microscopy techniques. Building on similar concepts in ultrafas...
متن کاملHigh-resolution low-dose scanning transmission electron microscopy.
During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording...
متن کامل