Pea choline kinase: purification, properties and isolation of a cDNA.
نویسندگان
چکیده
Choline kinase has been partially purified from pea seedlings and its properties studied. Using sequence information from soya bean and other choline kinases, we have also isolated a cDNA encoding the enzyme. It encodes a protein of 343 amino acids (calculated molecular mass of 39785 Da), which shows 82% homology with the soya bean choline kinase. The protein has been expressed in Escherichia coli with very good activity and high expression levels.
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 28 6 شماره
صفحات -
تاریخ انتشار 2000