Characterization of the Functional Domain of β2-Microglobulin from the Asian Seabass, Lates calcarifer
نویسندگان
چکیده
BACKGROUND β2-Microglobulin (β(2)M) is the light chain of major histocompatibility class I (MHC I) that binds non-covalently with the α heavy chain. Both proteins attach to the antigen peptide, presenting a complex to the T cell to be destroyed via the immune mechanism. METHODOLOGY/PRINCIPAL FINDINGS In this study, a cDNA sequence encoding β(2)M in the Asian seabass (Lates calcarifer) was identified and analyzed using in silico approaches to predict and characterize its functional domain. The β(2)M cDNA contains an open reading frame (ORF) of 351 bases with a coding capacity of 116 amino acids. A large portion of the protein consists of the IG constant domain (IGc1), similar to β(2)M sequences from other species studied thus far. Alignment of the IGc1 domains of β(2)M from L. calcarifer and other species shows a high degree of overall conservation. Seven amino acids were found to be conserved across taxa whereas conservation between L. calcarifer and other fish species was restricted to 14 amino acids at identical conserved positions. CONCLUSION/SIGNIFICANCE As the L. calcarifer β(2)M protein analyzed in this study contains a functional domain similar to that of β(2)M proteins in other species, it can be postulated that the β(2)M proteins from L. calcarifer and other organisms are derived from a common ancestor and thus have a similar immune function. Interestingly, fish β(2)M genes could also be classified according to the ecological habitat of the species, i.e. whether it is from a freshwater, marine or euryhaline environment.
منابع مشابه
Evaluation of potential Asian seabass (Lates calcarifer) culture in ponds of Choebde, Abadan
Asian seabass (Lates calcarifer) is a potential candidate species for rearing in pond system in Iran because of its fast growth rate, ability to adapt to varying environmental conditions and its demand in domestic as well as export markets. Hence, growth parameters, survival and body composition of Asian seabass (20.70±0.36 g) was investigated. Experiment was conducted in three ponds In Choebde...
متن کاملLength-Weight Relationship of Asian Seabass (Lates calcarifer Bloch, 1790) from Pichavaram Mangrove Wa- ters, South East Coast of India
The length-weight relationship of Asian seabass (Lates calcarifer Bloch, Centropomidae) was estimated for the first time based on a wild population; specimens were collected from Pichavaram mangrove waters during October 2003 to September 2004. A highly significant correlation between length and weight, as a relative condition changing in the course of the year, were particularly observed. Thes...
متن کاملEffect of cryopreservation on lipid composition and antioxidant enzyme activity of seabass (Lates calcarifer) sperm
Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...
متن کاملFatty acid profile of Tra Catfish (Pangasius hypophthalmus) compared to Atlantic Salmon (Salmo solar) and Asian Seabass (Lates calcarifer)
The fatty acid composition of farmed Tra catfish (Pangasius hypophthalmus) was determined and compared to farmed Atlantic salmon (Salmo solar) and to wild-caught Asian seabass (Lates calcarifer). Saturated fatty acids (SFA) were most abundant in catfish (42.6%) while salmon (37.2%) and seabass (39.0%) were rich in polyunsaturated fatty acids (PUFA). Tra catfish contains Docosahexaenoic acid (DH...
متن کامل