Effect of Methanolic Momordica charantia seed extract and Depot medroxyprogesterone acetate (DMPA) to quantity and quality of rat sperm

To determine the effectiveness of the methanolic Momordica charantia seed extract dosage and depot medroxyprogesterone acetate (DMPA) to develop a potential male contraceptive. Methods: The extract of M. charantia seeds is done every day for 8 weeks of 50 mg/100 kg body weight of rats. DMPA dose is 0.625 mg/100 g BW of rat intramuscularly and given once on the first day of treatment after the first oral administration of M. charantia seed extract. There are 6 treatment groups (3 treatment groups were given the extract of M. charantia and 3 aquades control group) and 5 replicates of each group. Group (1) Administration of the extract of M. charantia seeds for 4 hours or 0 week, (2) seed extract of M. charantia every day for 4 weeks, (3) seed extract of M. charantia every day for 8 weeks, (4) administration of aquades (control 1) for 4 hours or 0 week, (5) administration of aquades (control 2) per day for 4 weeks, (6) administration of aquades (control 3) for 8 weeks. The combination of these two materials can accelerate the decline in the production of progesterone (precursor testosteron) and ultimately reduce the quantity and quality of rat sperm. The results obtained are decreased number of sperm, motility, normal morphology and viability of sperm after administration of the methanol extract of Momordica charantia seeds+DMPA at the weeks-4 and 8 (p<0.05). Conclusion: The methanol extract of Momordica charantia seeds (50mg/100g BW of rat) + DMPA (0.625 mg/100g BW of rat) is very good and effective for materials developed into a male contraceptive in the future