3-Methylindole production is regulated in Clostridium scatologenes ATCC 25775.
نویسندگان
چکیده
AIMS 3-Methylindole (3-MI) is a degradation product of L-tryptophan and is both an animal waste malodorant and threat to ruminant health. Culture conditions influencing 3-MI production in Clostridium scatologenes ATCC 25775 were investigated. METHODS AND RESULTS Extracellular 3-MI levels in cells cultured in brain heart infusion (BHI) medium (pH 7.0) at 33 degrees C and 37 degrees C for 72 h were 907 +/- 38 and 834 +/- 121 micromol l(-1), respectively. Cells cultured in tryptone-yeast (TY) extract medium at 37 degrees C for 48 h produced 104 +/- 86 micromol l(-1) 3-MI; however, addition of 1 mmol l(-1) L-tryptophan failed to increase extracellular levels (113 +/- 50 micromol l(-1) 3-MI). Specific activity of indole acetic acid decarboxylase measured in BHI, TY and TY plus 1 mmol l(-1) tryptophan-grown cells displayed 35-, 33- and 76-fold higher levels than in semi-defined medium-grown cells. CONCLUSIONS When cultured in rich medium, at 33 degrees C or 37 degrees C and pH 7.0, Cl. scatologenes ATCC 25775 optimally produced 3-MI. Addition of L-tryptophan to medium did not lead to significant increases in extracellular 3-MI levels. Whole cell assays indicate growth in rich medium significantly up-regulated 3-MI production. SIGNIFICANCE AND IMPACT OF THE STUDY Information presented here may prove useful in understanding what factors influence 3-MI production in malodorous animal wastes.
منابع مشابه
Draft Genome Sequence of Clostridium scatologenes ATCC 25775, a Chemolithoautotrophic Acetogenic Bacterium Producing 3-Methylindole and 4-Methylphenol
Clostridium scatologenes ATCC 25775 is a strictly anaerobic and chemolithoautotrophic acetogenic bacterium that converts syngas into multi-carbon compounds such as acetate, indole, 3-methylindole, and 4-methylphenol. Here we report the draft genome sequence of C. scatologenes ATCC 25775 (7.3 Mbp) to elucidate its metabolic pathway for syngas fermentation.
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عنوان ژورنال:
- Letters in applied microbiology
دوره 48 1 شماره
صفحات -
تاریخ انتشار 2009