A comparative study of in vitro antioxidant and DNA damage protection of soxhlet vs microwave assisted extracts of Michelia champaca Linn. flowers

Present study was designed to compare antioxidant and DNA damage protection of Soxhlet assisted (80% methanol) extract (SEMC) with microwave assisted (80% methanol) extract of Michelia champaca Linn. (MEMC). The in vitro antioxidant activity of SEMC and MEMC were tested for DPPH, hydroxyl radical (OH) by deoxyribose, lipid peroxidation and reducing power capacity. The extracts were also tested for protection against hydroxyl radical and tertiary butylated peroxide (t-BOOH) radical mediated DNA damage. SEMC showed highest percentage of inhibition in all the concentrations in DPPH assay. Both SEMC and MEMC showed better percentage of inhibition 93.0 and 85.0%, respectively, at 50 μg/ml concentration in hydroxyl radical assay. In lipid peroxidation inhibition assay both extracts have shown good peroxidation inhibition activity. In reducing power assay SEMC showed highest power of reduction. MEMC exhibited good protection against hydroxyl radical and t-BOOH mediated damage to DNA.