A Review on 1,3-dipolar Cycloaddition Reactions in Bioconjugation and It’s Importance in Pharmaceutical Chemistry
نویسندگان
چکیده
1,3-dipolar Cycloaddition (DC) reactions have emerged as one of the important type of cycloaddition reactions from the view point of chemical and biological industry. Particularly the Azide-Alkyne Cycloaddition, also known as “Click Chemistry” has opened up new challenges in front of medicinal and pharmaceutical chemists. Bioconjugation is a technique of coupling two different biomolecules by covalent linkage, employed for synthesizing protein conjugates, immobilized enzymes and antibodies and also in purification of proteins. In the current work, a discussion is made with the mechanisms of Cu catalyzed Azide-Alkyne Cycloaddition (CuAAC) and Ru catalyzed Azide-Alkyne Cycloaddition (RuAAC) followed by their applicability in bioconjugation techniques. The CuAAC is already gained importance in bioconjugation while the RuAAC shows huge promise for the future.
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