A rapid chemical means for removing labile glycohemoglobin.
نویسندگان
چکیده
We have devised a rapid and convenient method of eliminating the contribution of labile glycosylated hemoglobin in the hemoglobin A1 assay. The procedure requires a 30-min incubation of erythrocytes in 30 mM semicarbazide and 12 mM aniline (pH 5, 38 degrees C). This chemical means of eliminating the labile fraction is as effective as a 14-h incubation in isotonic saline as measured with high-pressure liquid chromatography or electrophoresis methods. The removal of the labile glycosylated hemoglobin fraction preserves the assay as a reliable index of chronic metabolic control.
منابع مشابه
Fully automated assay of glycohemoglobin with the Abbott IMx analyzer: novel approaches for separation and detection.
We describe a novel assay for measuring glycohemoglobin directly from anticoagulated whole blood with the Abbott IMx analyzer. The glycohemoglobin is labeled with a soluble polyanionic affinity reagent and the anionic complex is then captured with a cationic solid-phase matrix. Glycohemoglobin is quantified by measuring the quenching by heme of the static fluorescence from an added fluorophore....
متن کاملMulticenter evaluation of Tosoh glycohemoglobin analyzer.
BACKGROUND We describe an Anglo-French evaluation of a new analyzer. METHODS The Tosoh HLC-723 GHb V, A1c2.2 glycohemoglobin analyzer is an HPLC instrument with primary blood tube sampling, bar-code reading, cap piercing, and the ability to chromatographically separate labile hemoglobin A1c (HbA1c). We evaluated two analytical protocols, 2.2 and 3.0 min, and compared results for blood samples...
متن کاملThe Effect of Swedish Massage on Glycohemoglobin in Children with Diabetes Mellitus
Objectives: Diabetes mellitus (DM) is the most common endocrine disease in children. Massage therapy can improve glucose metabolism in DM. This study was conducted to determine the effect of Swedish massage on the Glycohemoglobin (HbA1c) in children with DM. Methods: This study was an semi-experimental (clinical trial) conducted on thirty-six children, 6-12 years old with (DM), recruited fro...
متن کاملAcid-labile δ-ketal-β-hydroxy esters by asymmetric hydrogenation of corresponding δ-ketal-β-keto esters in the presence of CaCO3.
A series of acid-labile, optically pure ε-substituted δ-ketal-β-hydroxy esters were obtained by a Ru-SunPhos catalyzed asymmetric hydrogenation of the corresponding ε-substituted δ-ketal-β-keto esters. CaCO(3) played a dual role in the hydrogenation reaction--removing the acid generated during the formation of the catalyst and maintaining the activity of the catalyst.
متن کاملRapid method for eliminating labile glycosylated hemoglobin from the assay for hemoglobin A1.
The irreversible formation of stable glycosylated hemoglobin proceeds through a labile intermediate that is indistinguishable, by most methods, from the stable glycosylated product. The inclusion of the labile intermediate, which changes acutely with acute blood glucose changes, detracts from the utility of the assay as an index of chronic glucose concentration. We have developed a rapid, relia...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Diabetes
دوره 30 8 شماره
صفحات -
تاریخ انتشار 1981