Correction Ox40 Triggering Blocks Suppression by Regulatory T Cells and Facilitates Tumor Rejection
نویسنده
چکیده
The authors regret that two of the fl ow cytometry histograms in Fig. 3 C were inadvertently duplicated. The corrected fi gure and its legend appear below. Figure 3. Tumor-infi ltrating T reg cells express functional OX40. (A) TILs were purifi ed from a pool of CT26 tumor nodules, and the CD4 + cell population was analyzed by fl ow cytometry (percentages are shown). As controls, CD4 + T cells were purifi ed from the spleens of tumor-free mice (STF). (top) On gated CD4 + T cells, the expression of Foxp3 versus CD25 was evaluated. (bottom) Among CD4 + CD25 + T cells, Foxp3 versus OX40 expression is shown. (B) OX40 expression was evaluated on gated CD4 + Foxp3 + T cells from TILs of tumor-bearing mice. As controls, isotype staining on Foxp3 + TILs and OX40 staining on Foxp3 + CD4 + spleno-cytes from tumor-free mice are shown. (C) CD4 + CD25 Ϫ T cells (Teff), obtained from the spleens of tumor-free mice, were CFSE labeled and seeded either alone or combined at 1:1, 1:2, or 1:4 ratios with unlabeled CD4 + T cells purifi ed from TILs (T reg TIL) of tumor-bearing mice, either untreated or 6 h after intratumor OX86 injection (anti-OX40 in vivo). No major differences in the composition of tumor-infi ltrating CD4 + T cells or in Foxp3 expression were evident 6 h after treatment. As controls, CD4 + CD25 + T cells from spleens of tumor-free mice were used (T reg STF). T reg cells were either pretreated with anti-OX40 mAb in vitro or with rat IgG as mock control. After 72 h, effector T cells were tested for CFSE dilution as a marker of proliferation. Representative plots of one out of three independent experiments are shown and indicate percentages of proliferating (CFSE low) versus resting (CFSE high) effector T cells.
منابع مشابه
OX40 triggering blocks suppression by regulatory T cells and facilitates tumor rejection
Regulatory T (T reg) cells are the major obstacle to cancer immunotherapy, and their depletion promptly induces conversion of peripheral precursors into T reg cells. We show that T reg cells can be functionally inactivated by OX40 triggering. In tumors, the vast majority of CD4(+) T cells are Foxp3(+) and OX40(bright). However, intratumor injection of the agonist anti-OX40 monoclonal antibody (...
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