Elaboration of Bacillus anthracis antigens in a new, defined culture medium.
نویسندگان
چکیده
Improved culture conditions and a new, completely synthetic medium (R medium) were developed to facilitate the production of Bacillus anthracis holotoxin antigens. Levels of these antigens up to fivefold greater than the highest previously reported values were recovered with the described system. Cultures of Sterne, V770-NP1-R, and Vollum 1B strains of B. anthracis were monitored for growth, pH change, glucose utilization, supernatant protein concentration, lethal toxin activity, and protease activity.
منابع مشابه
Studies on immunity in anthrax. VII. Carbohydrate metabolism of Bacillus anthracis in relation to elaboration of protective antigen.
The elaboration in vitro of protective antigen of Bacillus anthracis in chemically defined media has been described in previous reports (Wright etal., 1954a; Puziss and Wright, 1954). The alumprecipitated antigen was effective in immunization of laboratory and domestic animals and well tolerated in man (Wright et al., 1954b; Schlingman et al., 1956; Jackson et al., 1957). The antigen was elabor...
متن کاملStudies on immunity in anthrax. XI. Control of cellular permeability by bicarbonate ion in relation to protective antigen elaboration.
Puziss, Milton (Fort Detrick, Frederick, Md.) and Mary B. Howard. Studies on immunity in anthrax. XI. Control of cellular permeability by bicarbonate ion in relation to protective antigen elaboration. J. Bacteriol. 85:237-243. 1963.-No elaboration of Bacillus anthracis protective antigen was demonstrated after addition of bicarbonate to cultures incubated 42 hr in a bicarbonate-free medium. Ant...
متن کاملAntibody titers of PEG-PLA block copolymer nanosphere containing chimeric recombinant protein of protective antigen and lethal factor of Bacillus anthracis
Introduction: To date, many vaccines have been developed for anthrax but not yet an ideal vaccine. In this study, chimeric protein containing domain 1 lethal factor and domain 4 protective antigens of Bacillus anthracis in copolymer nanocapsules were used to solve the problems caused by existing vaccines and to increase the efficiency of the proposed vaccine. Materials and Methods: In this expe...
متن کاملFurther purification studies on the protective antigen of Bacillus anthracis produced in vitro.
In a previous investigation (Strange and Belton, 1954), a protein fraction which was active in immunizing rabbits against artificially induced anthrax infection was isolated from culture filtrates of Bacillus anthracis. For the production of protective antigen, the synthetic medium of Wright et al. (1954) or a modified medium containing casein hydrolyzate (Belton and Strange, 1954) was used. No...
متن کاملDetermination of anthrax foci through isolation of Bacillus anthracis form soil samples of different regions of Iran
To isolate and detect anthrax spores form soil in different regions of Iran in order to find the anthrax foci‚ a total of 668 environmental specimens were collected during 2003-2004. Bacterial endospores were extracted from soil specimens via flotation in distilled water, incubation at room temperature, filtration, heat shock and culture on blood agar and selective PLET media. Bacillus anthraci...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Infection and immunity
دوره 39 1 شماره
صفحات -
تاریخ انتشار 1983