An Accelerated Approach to Sensitive ADA Assays
ثبت نشده
چکیده
The performance of an antibody in an assay often correlates with its affinity to the antigen. The ability to specifically select for antibodies on the basis of binding strength is therefore an important element of antibody assay development. Affinity or koff-rate determination can be carried out on several platforms; for example a rough ranking by ELISA, or quantitative screening using a technology such as surface plasmon resonance (SPR) or biolayer interferometry (BLI).
منابع مشابه
An Improved Flower Pollination Algorithm with AdaBoost Algorithm for Feature Selection in Text Documents Classification
In recent years, production of text documents has seen an exponential growth, which is the reason why their proper classification seems necessary for better access. One of the main problems of classifying text documents is working in high-dimensional feature space. Feature Selection (FS) is one of the ways to reduce the number of text attributes. So, working with a great bulk of the feature spa...
متن کاملAn Improved Flower Pollination Algorithm with AdaBoost Algorithm for Feature Selection in Text Documents Classification
In recent years, production of text documents has seen an exponential growth, which is the reason why their proper classification seems necessary for better access. One of the main problems of classifying text documents is working in high-dimensional feature space. Feature Selection (FS) is one of the ways to reduce the number of text attributes. So, working with a great bulk of the feature spa...
متن کاملAdenosine deaminase and terminal deoxynucleotidyl transferase: biochemical markers in the management of chronic myelogenous leukemia.
Serial determinations of adenosine deaminase (ADA) activity in 69 patients with chronic myelogenous leukemia provided a biochemical marker of disease activity. Eighty-nine % of patients in the accelerated phase had an elevation of ADA activity. This elevation was not a direct reflection of an increased absolute blast count. Furthermore, five of seven patients studied serially from the stable ph...
متن کاملDevelopment of AlphaLISA Technology Assays for the Detection of Neutralizing Antibodies in Human Serum
We have previously demonstrated the application of the AlphaLISA® technology for ADA screening and confirmatory assays.1 The usual step after the screening process is to characterize the ADA response by determining if the ADA could neutralize the activity of the drug. Generally, the ADA binds to the drug without affecting its efficacy. However, the presence of neutralizing ADA (NAb) is of great...
متن کاملFailure Process Modeling with Censored Data in Accelerated Life Tests
Manufacturers need to evaluate the reliability of their products in order to increase the customer satisfaction. Proper analysis of reliability also requires an effective study of the failure process of a product, especially its failure time. So, the Failure Process Modeling (FPM) plays a key role in the reliability analysis of the system that has been less focused on. This paper introduces a f...
متن کامل