Composition of mammalian ribosomal subunits: a re-evaluation.
نویسنده
چکیده
A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly employed assumptions about the relationship between the buoyant density of a particle in CsCl and its protein content are in error. The composition of ribosomal subunits cannot ordinarily be calculated from the buoyant density in CsCl.
منابع مشابه
Plant initiation factor 3 subunit composition resembles mammalian initiation factor 3 and has a novel subunit.
Eukaryotic initiation factor 3 (eIF3) is a multisubunit complex that is required for binding of mRNA to 40 S ribosomal subunits, stabilization of ternary complex binding to 40 S subunits, and dissociation of 40 and 60 S subunits. These functions and the complex nature of eIF3 suggest multiple interactions with many components of the translational machinery. Recently, the subunits of mammalian a...
متن کاملCharacterization of Staufen 1 ribonucleoprotein complexes.
In Drosophila oocytes and neuroblasts, the double-stranded RNA binding protein Staufen assembles into ribonucleoprotein particles, which mediate cytoplasmic mRNA trafficking and translation. Two different mammalian orthologues also appear to reside in distinct RNA-containing particles. To date, relatively little is known about the molecular composition of Staufen-containing ribonucleoprotein co...
متن کاملDrastic alteration of ribosomal RNA and ribosomal proteins in showdomycin-resistant Escherichia coli.
In a mutant of Escherichia coli resistant to showdomycin, both the 50S and 30S ribosomal subunits contain RNA species in which the purine concentration greatly exceeds that of pyrimidines. The same is true for total rapidly-labeled RNA. The modified ribosomal RNA hybridizes poorly with homologous DNA, which is apparently unchanged in base composition. Acrylamide gel electrophoresis of mutant ri...
متن کاملP-152: Expression of Recombinant Human Luteinizing Hormone (hLH) in CHO Cells
Background: Human luteinizing hormone (hLH) stimulates steroid biosynthesis of the ovary, triggers ovulation and prepares androgen production of testicular Leydig cells. LH belongs to the family of glycoprotein hormones that are heterodimers consisting of a common α-subunit and specific β-subunit. hLH is necessary for clinical and infertility treatment. Recombinant DNA technology provides a use...
متن کاملEvaluation of Unit's Performance in Presence of Subunits by Using GDEA
Data Envelopment Analysis (DEA) is a technique that uses all collected observations to measure performance. This method presents no data about how to operate on DMU. The present research attempted to study a unit with all its subunits, if the unit is efficient, it means that all its subunits are efficient too and if it is an inefficient, it shows clearly that which one of the subunits makes ine...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 71 4 شماره
صفحات -
تاریخ انتشار 1974