Roles of ERβ and GPR30 in Proliferative Response of Human Bladder Cancer Cell to Estrogen
نویسندگان
چکیده
Bladder cancer belongs to one of the most common cancers and is a leading cause of deaths in our society. Urothelial carcinoma of the bladder (UCB) is the main type of this cancer, and the estrogen receptors in UCB remain to be studied. Our experiment aimed to investigate the possible biological effect of 17β-estradiol on human bladder-derived T24 carcinoma cells and to indicate its related mechanisms. T24 cells were treated with various doses of 17β-estradiol, and cell proliferation was detected using MTT assays. 17β-estradiol promoted T24 cell proliferation independent of ERβ/GPR30-regulated EGFR-MAPK pathway, while it inhibited cell growth via GPR30. Furthermore, the expression levels of downstream genes (c-FOS, BCL-2, and CYCLIN D1) were increased by 17β-estradiol and this effect was independently associated with activity of the EGFR-MAPK pathway. The two estrogen receptors might be potential therapeutic targets for the treatment of bladder cancer.
منابع مشابه
Response to “Comment on ‘Effects of Atrazine on Estrogen Receptor α– and G Protein–Coupled Receptor 30–Mediated Signaling and Proliferation in Cancer Cells and Cancer-Associated Fibroblasts’”
Albanito et al. recently reported that the herbicide atrazine was able to exert an estrogen-like proliferative activity in various cell models, including ovarian and breast cancer cells and cancer-associated fibroblasts, by inducing the expression of several estrogen target genes without binding to or activating the classical estrogen receptor (ER) α. They also showed that G protein–coupled rec...
متن کاملComment on “Effects of Atrazine on Estrogen Receptor α– and G Protein–Coupled Receptor 30–Mediated Signaling and Proliferation in Cancer Cells and Cancer-Associated Fibroblasts”
Albanito et al. recently reported that the herbicide atrazine was able to exert an estrogen-like proliferative activity in various cell models, including ovarian and breast cancer cells and cancer-associated fibroblasts, by inducing the expression of several estrogen target genes without binding to or activating the classical estrogen receptor (ER) α. They also showed that G protein–coupled rec...
متن کاملExpression of G protein-coupled estrogen receptor in gastric cancer tissues
Objectives: Estrogen possesses important functions in gastric carcinogenesis. However, the expression of G protein-coupled estrogen receptor (GPR30) in gastric cancer (GC) has never been investigated. This study aimed to compare the expression of GPR30 and estrogen nuclear receptors in GC tissues, matched tumor-adjacent tissues and normal gastric mucosa. Methods: Gastric mucosal biopsies were o...
متن کاملGPR30 regulates the EGFR-Akt cascade and predicts lower survival in patients with ovarian cancer
UNLABELLED OBJECTIVES G protein-coupled receptor 30 (GPR30) is a 7-transmembrane estrogen receptor that functions alongside traditional estrogen receptors to regulate the cellular responses to estrogen. Recent studies suggest that GPR30 expression is associated with a poor prognosis, and that this is due to the GPR30-mediated transactivation of the EGFR in breast cancer. However, the biologi...
متن کاملIdentification of Estrogen Receptor Dimer Selective Ligands Reveals Growth-Inhibitory Effects on Cells That Co-Express ERα and ERβ
Estrogens play essential roles in the progression of mammary and prostatic diseases. The transcriptional effects of estrogens are transduced by two estrogen receptors, ERα and ERβ, which elicit opposing roles in regulating proliferation: ERα is proliferative while ERβ is anti-proliferative. Exogenous expression of ERβ in ERα-positive cancer cell lines inhibits cell proliferation in response to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
دوره 2015 شماره
صفحات -
تاریخ انتشار 2015