In Vitro Anthelmintic Activity of Phyllanthus Niruri Linn. against Paramphistomes

Introduction: The perennial herb Phyllanthus niruri, belonging to the family Euphorbiaceae is commonly known as Bhuiavala in Marathi and possess antibacterial, analgesic, antipyretic, anti-hepatotoxic, antifungal and anthelmintic activity. Objective: The present study was carried out to validate the in vitro anthelmintic activity of hot aqueous whole plant extract of Phyllanthus niruri against Paramphistomes by petri-dish method. Materials & method: The in vitro trials for anthelmintic activity of aqueous whole plant extract of Phyllanthus niruri was conducted on mature live Paramphistomes by petri-dish method. The extracts were used at the concentration of 5 and 10 mg/ml and observation were made on their viability at room temperature. Result: The hot aqueous whole plant extract of Phyllanthus niruri was found to cause cessation of motility (paralysis) of Paramphistomes after 2:15 and 1:45 hours of exposure while the complete caesation of motility (death) was observed after 4:35 and 4:15 hours of exposure in the concentration of 5 and 10 mg/ml respectively. Conclusion: The hot aqueous whole plant extract of Phyllanthus niruri exhibited significant in vitro anthelmintic activity in a dose dependant manner.