DNP induced oxidative stress on blood components ameliorated by Pyrrole derivative of Tinospora cordifolia

Article history: Received on: 10/09/2016 Revised on: 09/10/2016 Accepted on: 22/10/2016 Available online: 20/01/2017 The intoxications and deaths caused by 2, 4-dinitrophenol (DNP) exposure through drugs, food supplements have been frequently reported and no specific treatment has been implemented to the toxic effects of DNP in adults and children. In this study, the purified compound (TCCP), a bioactive molecule from Tinospora cordifolia exhibited radical scavenging activities. Hence, we investigated the in vitro effect of TCCP on oxidative stress induced by DNP. Blood samples from healthy donors were treated with DNP (100μM/ml) for 2h. The DNP induced oxidative damage and effect of TCCP pre-treatment were determined by assessing the serum levels of cytoplasmic, lysosomal and oxidative stress markers. TCCP effectively inhibited DNP-induced oxidative stress on blood components, which was evidenced by the levels of endogenously generated reactive oxygen species and hydrogen peroxide. In addition, the depletion of GSH (reduced glutathione) content in DNP-treated cells was prevented by TCCP. Also, experimental results reflected the decreased expression of inflammation marker such as lipid peroxidase, catalase, LDH (lactate dehydrogenase), and phosphatase levels in cells with TCCP pre incubated DNP treated group. Therefore, these studies suggest the use of TCCP as an antioxidant and anti-inflammatory agent.