A rapid simplified method for plasma 25-hydroxyvitamin D estimation.
نویسنده
چکیده
منابع مشابه
Assay of vitamins D2 and D3, and 25-hydroxyvitamins D2 and D3 in human plasma by high-performance liquid chromatography.
I describe a new assay that is capable of measuring vitamin D2, vitamin D3, 25-hydroxyvitamin D2, and 25-hydroxyvitamin D3 in 2 ml of plasma or serum. Plasma is extracted by the Bligh and Dyer technique [Can. J. Biochem. Physiol. 37, 911 (1959)], the lipid component is fractionated by two high-performance liquid-chromatographic systems based upon adsorption and reversed-phase chromatography, an...
متن کاملSPE and LC-MS/MS Method for the Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 from Human Plasma
A liquid chromatography tandem mass spectrometry method for 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 from human plasma has been developed. Sample preparation was fast and efficient using a Thermo ScientificTM SOLATM hydrophobic reversed phase (HRP) plate. Analysis was carried out using a Thermo ScientificTM SyncronisTM C18 1.7 μm, 50 × 2.1 mm column to give a fast separation with a cycle t...
متن کاملAssessment of plasma 25-hydroxyvitamin D response to ultraviolet irradiation over a controlled area in young and elderly subjects.
1. The response of 25-hydroxyvitamin D [25(0H)Dl to artificial ultraviolet irradiation applied to a known area of dorsal skin was investigated in 18 subjects, small quantities of ultraviolet energy being used. Ultraviolet irradiation was administered on days, 1,3,5,8, 10, 12, 15 and 17, a total of 15 min being delivered over this time. In 15 subjects plasma 25-(0H)D showed a significant increas...
متن کاملAssay for plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 by "high-performance" liquid chromatography.
We describe a new, rapid, and simple column-chromatographic procedure for 25-OH vitamin D3 in plasma. The vitamin is extracted by use of Sep-pak C18 (octadecyl alkylated silicic acid), a short factory-packed reversed-phase column, and 25-OH vitamin D2 + 25-OH vitamin D3 fraction is eluted with methanol/water. The 25-OH D2 and 25-OH D3 are then well resolved on a high-resolution 3-micrometer sil...
متن کاملA fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS.
BACKGROUND Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. METHODS Sample preparation was based on preci...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 33 2 شماره
صفحات -
تاریخ انتشار 1980