Clinical determination of methemalbumin.

Methemalbumin interference in various biochemical assays of plasma.

During intravascular hemolysis and digestion of extravasated blood, heme from hemoglobin is oxidized to hematin, hich combines with plasma proteins to form methemalbu(1-4). One case of strong methemalbuminemia (140 oI/L) associated with intravascular hemolysis has been bserved in a patient after placing of an intracardiac valve. e plasma of this patient was so brown that we became terested in t...

N-of-1 Clinical Trials: Removing the Hay to Find the Needle.

Significant intravascular hemolysis can complicate dialysis (e.g., because of a kink in a dialysis line) and may result in the formation of methemalbumin (1 ). This gives serum a brown appearance, quite distinct from the typical reddish color associated with extravascular hemolysis. In this case, spectrophotometry performed before and after the addition of a reducing agent (sodium dithionite) (...

Methemalbumin. Ii. Effect of Pamaquine and Quinine on Pathways of Hemoglobin Metabolism.

Methods for measuring plasma hemoglobin in micromolar concentration compared.

Of eight methods examined for measuring plasma hemoglobin in micromolar concentration, all exhibited acceptable linearity, reproducibility, and concurrence except when specimens were icteric or lipemic or contained methemoglobin or methemalbumin. Measurement of absorbance at 578 nm with an Allen correction permits precise assay of plasma oxyhemoglobin concentration as low as 0.01 g/L (1 mg/dL, ...

Recent trends in designer drug abuse.

Methemoglobin, myoglobin, and methemalbumin can cause abnormal dark color in patient samples. “Lipemia” was measured at 660and 700-nm wavelengths (1 ); therefore any compounds that absorb light in this range will result in an increased lipemic index. In this patient, methemoglobin was always 7.5% (cooximetry), myoglobin was not detected (gel filtration chromatography and mass spectrometry), but...