X - ray crystallographic studies on Mlc and Aes , two transcriptional modulators from Escherichia coli
نویسنده
چکیده
Mlc is a prokaryotic transcriptional repressor controlling the expression of a number of genes encoding enzymes of the Escherichia coli phospho-transferase system (PTS), as well as manXYZ and malT, the gene of the global activator of the mal regulon. The mlc gene containing the point mutation R52H has been cloned into a pQE vector and the recombinant protein was expressed and purified as the selenomethioninelabeled derivative. Crystallization of the SeMet-Mlc was carried out using the vapourdiffusion method. The crystals obtained belong to the monoclinic space group C2 with the unit cell parameters a=235.95 Å, b = 74.71 Å, c = 154.95 Å, β = 129.15° and diffract to 2.9 Å resolution. Crystallization of Mlc from E. coli 30
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