Molecular characterization and differential expression of the myostatin gene in channel catfish (Ictalurus punctatus).

نویسندگان

  • Arif M Kocabas
  • Huseyin Kucuktas
  • Rex A Dunham
  • Zhanjiang Liu
چکیده

Myostatin is a recently discovered gene that inhibits muscle growth. In the present study, we characterized the myostatin locus and its expression in channel catfish (Ictalurus punctatus). The genomic DNA and cDNA encoding the channel catfish myostatin were cloned and sequenced. The myostatin gene has three exons encoding a protein of 389 amino acids. Comparison of the genomic sequences with those of the cDNA revealed that the myostatin cDNA was 1673 base pair (bp) long with a 5'-untranslated region (UTR) and 3'-UTR of 180 and 323 bp, respectively. The deduced amino acid sequences of the catfish myostatin is highly conserved with those of other organisms. The myostatin locus is highly polymorphic in channel catfish because of the presence of several microsatellites and single nucleotide polymorphic sites. The myostatin gene was expressed in various tissues and developmental stages at differential levels, suggesting complex regulation of this gene and perhaps roles for myostatin in addition to those originally suggested.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Cloning and characterization of myogenic regulatory genes in three Ictalurid species.

We report sequence, tissue expression and map-position data for myogenin, MYOD1, myostatin and follistatin in three Ictalurid catfish species: channel catfish (Ictalurus punctatus), blue catfish (I. furcatus) and white catfish (Ameiurus catus). These genes are involved in muscle growth and development in mammals and may play similar roles in catfish. Amino acid sequences were highly conserved a...

متن کامل

Sequence and expression of a cDNA encoding both pituitary adenylate cyclase activating polypeptide and growth hormone-releasing hormone-like peptide in channel catfish (Ictalurus punctatus).

In nonmammalian vertebrates, pituitary adenylate cyclase activating polypeptide (PACAP) and a putative growth hormone-releasing hormone (GHRH-like peptide) are encoded by a single mRNA transcript. Both PACAP and GHRH have been implicated in the control of fish growth. Although the gene encoding PACAP and GHRH-like peptide (GHRHLP) has been cloned in other fishes, characterization of this gene i...

متن کامل

Lipopolysaccharide regulates myostatin and MyoD independently of an increase in plasma cortisol in channel catfish (Ictalurus punctatus).

The effects of lipopolysaccharide (LPS) on plasma cortisol and the expression of MyoD and myostatin (MSTN) mRNAs were evaluated in channel catfish. In addition, the effect of dexamethasone (Dex) on MyoD and MSTN mRNAs was examined. For the LPS injection experiments, juvenile channel catfish were injected intraperitoneally with 1.5 mg/kg LPS or sterile PBS. Blood was collected at 1, 3, 12, and 2...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochimica et biophysica acta

دوره 1575 1-3  شماره 

صفحات  -

تاریخ انتشار 2002