Lamblia Intestinalis and Poona Diarrhœa

Rapid tagging and integration of genes in Giardia intestinalis.

We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.

PCR amplification of triosephosphate isomerase gene of Giardia lamblia in formalin-fixed feces.

Giardia lamblia (syn G. intestinalis, G. duodenalis) is the intestinal protozoan producing non-bacterial diarrhea most prevalent in the world. PCR genotype classification of Giardia in feces depends on the quality and quantity of purified DNA and the removal of a great number of inhibitors. The aim of this study was to adapt a PCR protocol to the amplification of the triosephosphate isomerase (...

Confirmed detection of Cyclospora cayetanesis, Encephalitozoon intestinalis and Cryptosporidium parvum in water used for drinking.

Human enteropathogenic microsporidia (HEM), Cryptosporidium parvum, Cyclospora cayetanesis, and Giardia lamblia are associated with gastrointestinal disease in humans. To date, the mode of transmission and environmental occurrence of HEM (Encephalitozoon intestinalis and Enterocytozoon bieneusi) and Cyclospora cayetanesis have not been fully elucidated due to lack of sensitive and specific envi...

Prevalence of Entamoeba histolytica and Giardia lamblia infection in a Rural Area of Haryana, India

An intestinal parasitic infestation is major public health problem in the world. It is estimated that 3.5 billion people are involved globally and 450 millions are suffering as a result of these infections, majority being children (WHO, 2000). The most important protozoan parasites are the Entamoeba histolytica and Giardia lamblia. Giardia lamblia (syn G. intestinalis, G.duodenalis) is one of t...

Fermentation enzymes of Giardia intestinalis , pyruvate : ferredoxin 3 oxidoreductase and hydrogenase , do not localize to its mitosomes