Potential Competency of Glucose Oxidase for Modification of Flower Colour in Carthamus tinctorius

نویسنده

  • Koshi Saito
چکیده

Glucose was found to be a potential stimulator o f carthamin formation in triturated florets o f C. tinctorius. At a 10 m M glucose level, carthamin-producing activity was raised from 3.2fold (upper) to 2.0-fold (lower) level compared with the control having no sugar. Glucose oxi­ dase (ß-D-glucose: oxygen 1-oxidoreductase, EC 1.1.3.4) from Aspergillus niger could catalyze the conversion of precarthamin to carthamin, indicative o f flower colour modification in C. tinctorius. For optimum reaction, precarthamin, ß-D-glucose, and oxygen were required. N o manganese enhanced the catalytic enzyme process.

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تاریخ انتشار 2013