Integration of Agglutination Assay for Protein Detection in Microfluidic Disc Using Blu-ray Optical Pickup Unit and Optical Fluid Scanning
نویسندگان
چکیده
We present a novel strategy for thrombin detection by combining a magnetic bead based agglutination assay and low-cost microfluidic disc. The detection method is based on an optomagnetic readout system implemented using a Blu-ray optical pickup unit (OPU) as main optoelectronic component. The assay, from sample to answer, is fully integrated on a microfluidic disc which embeds on-disc mixing ensuring full automation of the assay along with less sample-to-answer time compared to similar methods. Moreover, we compare the optomagnetic readout to the cluster size distribution determined using a commercial optical scanning imaging instrument.
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