Optimization of microculture and evaluation of miniculture for the isolation of Leishmania parasites from cutaneous lesions in Peru.

Evaluation of a microculture method for isolation of Leishmania parasites from cutaneous lesions of patients in Peru.

Traditional culture of Leishmania spp. is labor intensive and has poor sensitivity. We evaluated a microculture method for the diagnosis of cutaneous leishmaniasis in consecutive patients presenting to the Leishmaniasis Clinic at the Instituto de Medicina Tropical Alexander von Humboldt, Peru, for evaluation of skin lesions. Lesion aspirates were cultured in duplicate and parallel in traditiona...

EVALUATION OF TWO BIPHASIC CULTURE MEDIA FOR ISOLATION OF LEISHMANIA PARASITES FROM CUTANEOUS ULCERS IN IRANIAN PATIENTS

Sporotrichoid Cutaneous Leishmaniasis in Central Iran

More than 90% of patients infected with cutaneous leishmaniasis live in Afghanistan, the Middle East, Algeria, Brazil, and Peru. Cutaneous leishmaniasis in Iran is caused by Leishmania tropica and Leishmania major. Most skin lesions evolve from papular to nodular, to ulcerative form with a central depression surrounded by a raised indurate border. Some lesions persist as nodules or plaques. Mul...

The effect of different concentrations of topical podophyllin on cutaneous leishmaniasis

Introduction: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by Leishmania species. Currently accessible treatments remain insufficient, and there is pressure to develop suitable and effectual options. In this study, we used different concentrations of podophyllin in vitro on leishmania parasites and then on leishmaniasis lesions in mice and compared their efficacy. Method: We used ...

Evaluating the Coconut Water as a Replacement for the Fetal Calf Serum in Cultivation of ‎Promastigotes of Leishmania Infantum

Backgrounds and Aims: The protozoan parasites of the genus Leishmania can be stated as the causative agents of the various clinical diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, which is the most expensive ingredient in these media. The coconut water is packed with nutrients that yield an array of health benefits. Materials and Methods: In the present st...