A Cellulolytic Enzyme Preparation from Myrothecium Verrucaria
نویسندگان
چکیده
Cellulolytic enzyme preparations active against various forms of cellulose have been reported by a number of investigators who have used a variety of different sources (8). Fungi which have been employed for the preparation of such extracts are Sporotrichum cards (15), Aspergillus oryxae (3,2), Merulius lachrymans (9), and Coniophora cerebella (7), while bacteria have included Bacterium protoxoides (5), Bacterium bosporum (5), Cellulobacillus myxogenes (14), Cellulobacillus mucosus (14), Plectridium cellulolyticum (lo), several species of Cytophaga (l), and various thermophilic cellulose-decomposing bacteria (11). In addition, active cellulolytic extracts have been prepared from the intestinal juice of the snail Helix pomatia (6), from green malt (12), and from the protozoon Eudiplodinium neglectum which was isolated from cattle rumen (4). Although the data presented by several of these investigators are certainly suggestive of true cellulolytic enzyme activity, nevertheless these workers in general have not conclusively established the cell-free nature of their preparations. This is of considerable importance in view of failure of other investigators to obtain cell-free cellulolytic enzymatic preparation from fungi. Questions were raised as to the possibility of bacterial or fungal contamination during the period (usually several days or more) of testing for cellulolytic activity. The present paper describes the preparation and some of the properties of a cell-free cellulolytic enzyme from Myrothecium verrucaria, which has been shown to be one of the most active of the cellulose-destroying fungi isolated from deteriorated fabrics (16).
منابع مشابه
A cellulolytic enxyme preparation from Myrothecium verrucaria.
Cellulolytic enzyme preparations active against various forms of cellulose have been reported by a number of investigators who have used a variety of different sources (8). Fungi which have been employed for the preparation of such extracts are Sporotrichum cards (15), Aspergillus oryxae (3,2), Merulius lachrymans (9), and Coniophora cerebella (7), while bacteria have included Bacterium protoxo...
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