Diffusate media for cultivation of oral anaerobic bacteria.

Antigen-free media suitable for cultivating anaerobic bacteria from the human gingival crevice were devised, based upon supplementing diffusates from standard media ingredients. Growth in these is at least as good as in customary media. Formulations suitable for the major species encountered are described and compared. 概要:口 腔内嫌気性培養用に考案 した透析培地について,真 下 彰*,S・A・ エ リソン*.口 腔内諸 疾患 の免疫学的研究 を,と くに 口内細菌 の抗原性について しらべた いときに,そ の培養基 に由来す る抗 原物質 にいろいろ と妨害 され る.一 方多 くの口腔 細菌 は理想的 な合成培地 に,な かなか うま く発育 して くれない。そこで,抗 原物質をふ くまないで,一 定 の培養後培地内容物質 を全部透折 で除 くことが出来 るい くつかの培地 を考案 し,他 に応用 して好成績 を得 てい るのでその処方 と代表的な結果 を報告す る. The growth requirements for the several species of anaerobic bacteria resident in the gingival crevice are largely unknown12). A few instances can be noted in which addition of specific chemicals to standard media bases has shown the need for supplementation of these media in order to obtain good growth, e.g. the apparent requirement for hemin and menadione, by some types of Bacteroides3). The available media are correspondingly complex. Simple defined media are a great convenience, almost a necessity for immunologic studies of bacteria. An alternative which has often been satisfactory is to employ diffusates7) prepared from standard media. Since our present work was particularly concerned with soluble antigensof oral bacteria, it was decided to attempt to develop a series of such media using which we might obtain consistent growth of the organisms under study. Materials and Methods 1. General The bacterial strains tested are listed by genus in Table 1. All strains except those of Corynebacteria were obligately anaerobic. The organisms were isolated from the indicated sources by streaking into nutrient agar with 10% sheep blood. Bacteroides melaninogenicus (BM) was obtained from Dr. Ronald Gibbons, Forsyth Dental Center , Boston. When growth was adequate the colonies were transfered into blood agar plates and restreaked to check purity. Spirochetes were isolated into S-3 medium (see below). Inocula were placed 38 * Department of Oral Biology School of Dentistry, State University of New York at Buffalo (Buffalo , New York 14226) * ニ ュ ー ヨー ク州 立 バ フ ァ ロー大 学歯 学部 口腔 生 物 学 教室 昭 和46年12月1日 受 付