Eight new restriction endonucleases fröm Herpetosiphon giganteus--divergent evolution in a family of enzymes.
نویسندگان
چکیده
Characterization of eight restriction endonucleases isolated from five strains of Herpetosiphon giganteus is described. HgiCI from strain Hpg9 recognizes and cleaves the degenerate sequence: GGPyPuCC, producing 5'-hexanucleotide protruding ends. Endonucleases HgiBI, HgiCII and HgiEI are isoschizomers of AvaII; HgiCIII and HgiDII are isoschizomers of SalI; and HgiDI and HgiGI are isoschizomers of AcyI. Based upon their closely related and in part overlapping recognition specificities a close evolutionary relationship is proposed for all known Hgi restriction endonucleases.
منابع مشابه
Restriction endonucleases from Herpetosiphon giganteus: an example of the evolution of DNA recognition specificity?
We describe the partial purification and characterisation of five Type II restriction endonucleases from two strains of Herpetosiphon giganteus. One of the activities, HgiJII, was the first enzyme found that cleaves DNA at the family of related sequences 5'-G-R-G-C-Y/C-3'. This enzyme may be related to the enzyme HgiAI from a different strain of the same species, and which cleaves at the sites ...
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 12 7 شماره
صفحات -
تاریخ انتشار 1984