Densitometric Analysis of Alkaline Phosphatase in the Duodenal and Jejunal Enterocytes of 8-week-old Piglets with Retarded Growth
نویسندگان
چکیده
The activity of alkaline phosphatase in the duodenal and jejunal enterocytes was determined in 8-week-old clinically healthy piglets with normal (12 animals, average body weight 15.30 + 0.49 kg) and retarded growth (12 animals, average body weight 5.30 + 0.75 kg). A densitometric analysis was used. A marked decrease in an average density of the alkaline phosphatase (P < 0.001) was observed in the animals with retarded growth. This was in contrast with the results from piglets with normal growth. The obtained results point to a relationship between the amount of the investigated enzyme found in the microvillous zone of enterocytes and the functional state of enterocytes.
منابع مشابه
Early weaning reduces small intestinal alkaline phosphatase expression in pigs.
Expression of the small intestinal alkaline phosphatase (IAP) is enterocyte differentiation dependent and plays essential roles in the detoxification of pathogenic bacterial lipopolysaccharide endotoxin, maintenance of luminal pH, organic phosphate digestion, and fat absorption. This study was conducted to examine the effect of early weaning on adaptive changes in IAP digestive capacity (V(cap)...
متن کاملFunctional development of small intestine of Japanese quail hatched on MIR orbital station.
The effect of microgravity on functional development of the small intestine of Japanese quails incubated for 2-3 d and hatched on the orbital station MIR was examined. After 5 d of space flight duodenal and jejunal alkaline phosphatase (AP) activity of the experimental group was compared with the AP activity in quails of the same age hatched on the Earth (laboratory controls). Short-term microg...
متن کاملClaudin-4 Undergoes Age-Dependent Change in Cellular Localization on Pig Jejunal Villous Epithelial Cells, Independent of Bacterial Colonization
Newborn piglets are immunologically naïve and must receive passive immunity via colostrum within 24 hours to survive. Mechanisms by which the newborn piglet gut facilitates uptake of colostral cells, antibodies, and proteins may include FcRn and pIgR receptor-mediated endocytosis and paracellular transport between tight junctions (TJs). In the present study, FcRn gene (FCGRT) was minimally expr...
متن کاملEffect of administration of isatin on alkaline phosphatase of various organs of rat.
In vivo administration of isatin (200 mg/kg) significantly lowered the activity of rat kidney alkaline phosphatase after 5 hr but enhanced the activity of rat duodenal and jejunal enzyme after 2 and 5 hr (P less than 0.01). The increased activity of the duodenal and jejunal alkaline phosphatase might be due to the induction of the enzyme by isatin.
متن کاملAnalytical subcellular fractionation studies on enterocytes from the jejunum and ileum of the rat and some properties of brush-border alkaline phosphatase.
1. Enterocytes, isolated from the proximal jejinum and distal ileum of the rat, were homogenized and their organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal organelles, RNA and protein were determined in the sucrose gradients and related to the activities per entercocyte. 2. In the jejunum the modal eq...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003