Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.
نویسنده
چکیده
Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.
منابع مشابه
Isolation and collection of two populations of viable sperm cells from the pollen of Plumbago zeylanica.
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ورودعنوان ژورنال:
- Plant physiology
دوره 81 1 شماره
صفحات -
تاریخ انتشار 1986