Bacterial synthesis of a novel human leukocyte interferon.

نویسندگان

  • E Yelverton
  • D Leung
  • P Weck
  • P W Gray
  • D V Goeddel
چکیده

A novel human leukocyte interferon cDNA clone (LeIF B) was identified in a cDNA library prepared using polyadenylated mRNA of a myeloblastoid cell line. The nucleotide sequence of LeIF B differs significantly from other published leukocyte interferon cDNA sequences. An expression plasmid was constructed which directs the synthesis in E. coli of 8 x 10(7) interferon units per liter of culture. LeIF B exhibits markedly different specificities from another bacterially synthesized human leukocyte interferon, LeIF A.

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عنوان ژورنال:
  • Nucleic acids research

دوره 9 3  شماره 

صفحات  -

تاریخ انتشار 1981